Publication - RNAi Using a Chitosan,siRNA Nanoparticle System: In Vitro and In Vivo Applications

RNAi Using a Chitosan,siRNA Nanoparticle System: In Vitro and In Vivo Applications

Research Area:	Nucleic Acid Technology	Year:	2009
Type of Publication:	Article
Authors:	M. O. Andersen K. A. Howard J. Kjems
Journal:	Methods Mol Biol	Volume:	555
Pages:	77-86


Note:
RNAi Using a Chitosan,siRNA Nanoparticle System: In Vitro and In Vivo Applications
Abstract:
Delivery is a key issue in development of clinically relevant RNAi therapeutics. Polymeric nanoparticles formed by self-assembly of polycations with siRNA can be used for extracellular delivery, cellular uptake and intracellular trafficking as a strategy to improve the therapeutic potential of siRNA. This chapter describes a chitosan-based nanoparticle system for in vitro and in vivo transfection of siRNA into cells. The method exploits the mucoadhesive and mucopermeable properties of this cationic polysaccharide to deliver siRNA across mucosal epithelium and provides a platform for targeting human diseases with RNAi therapeutics.
Digital version

[ Back ]

CDNA highlights

Single-molecule chemical reactions on DNA origami

Researchers at the Danish National Research Foundation’s Centre for DNA Nanotechnology (CDNA) at iNANO demonstrate that it is possible to control chemical reactions on DNA nanostructures and generate images of reactions of individual molecules. The results were published on 28 February in Nature Nanotechnology. The article is available here

CDNA news

May 2010: DNA nano-spider published in Nature

CDNA collaborator Hao Yan publishes the paper "Molecular robots guided by prescriptive landscapes". A molecular nanorobot dubbed a "spider" and labeled with green dyes traverses a substrate track built upon a DNA origami scaffold. It journeys towards its red-labeled goal by cleaving the visited substrates, thus exhibiting the characteristics of an autonomously moving, behavior-based robot at the molecular scale.

In the same issue of Nature DNA origami was used to create an assembly line for synthesizing gold nanoparticles.