| |
|
|
| |
|
Wednesday, 06 May 2009 23:18 |
Self-assembly of a nanoscale DNA box with a controllable lid Nature 459, 73-76 (7 May 2009) Ebbe S. Andersen, Mingdong Dong, Morten M. Nielsen, Kasper Jahn, Ramesh Subramani, Wael Mamdouh, Monika M. Golas, Bjoern Sander, Holger Stark, Cristiano L. P. Oliveira, Jan Skov Pedersen, Victoria Birkedal, Flemming Besenbacher, Kurt V. Gothelf, and Jørgen Kjems The unique structural motifs and self-recognition properties of DNA can be exploited to generate self-assembling DNA nanostructures of specific shapes using a 'bottom-up' approach. Several assembly strategies have been developed for building complex three-dimensional (3D) DNA nanostructures. Recently, the DNA 'origami' method was used to build two-dimensional addressable DNA structures of arbitrary shape that can be used as platforms to arrange nanomaterials with high precision and specificity. A long-term goal of this field has been to construct fully addressable 3D DNA nanostructures. Here we extend the DNA origami method into three dimensions by creating an addressable DNA box 42 x 36 x 36 nm3 in size that can be opened in the presence of externally supplied DNA 'keys'. We thoroughly characterize the structure of this DNA box using cryogenic transmission electron microscopy, small-angle X-ray scattering and atomic force microscopy, and use fluorescence resonance energy transfer to optically monitor the opening of the lid. Controlled access to the interior compartment of this DNA nanocontainer could yield several interesting applications, for example as a logic sensor for multiple-sequence signals or for the controlled release of nanocargos. Releated content at Nature:
University news
Science newsMediaBlogsDiscussions Other Databases
|
|
|
| |
|
|
|
| |
|
|
| |
CDNA highlights
 March 2, 2010
Single-molecule chemical reactions on DNA origami Researchers at the Danish National Research Foundation’s Centre for DNA Nanotechnology (CDNA) at iNANO demonstrate that it is possible to control chemical reactions on DNA nanostructures and generate images of reactions of individual molecules. The results were published on 28 February in Nature Nanotechnology. The article is available here |
|
Read more... |
|
|
|
| |
CDNA news
 February 12, 2010
Electrochemical assay for attomole detection of DNA The project was carried out in collaboration between CDNA and DTI. We combined a magnetic bead sandwich hybridization capture assay used for pre-concentration and bioseparation of target DNA, with a lipase-based amplification and electrochemical readout system. The signal amplification in the DNA assay is based on the catalytic activity of a lipase enzyme, and this “electrochemical blotting” concept is principally new. The use of this hydrolytic enzyme allowed for close to few-molecule detection of lipase-labeled DNAs at the electrode surface, due to accumulation of the catalysis product (ester bond cleavage and removal of the redox label from the zone of electrochemical reaction). The developed electrochemical lipase- and magnetic beads-based sandwich hybridization assay represents a fundamentally new electrochemical approach for sensitive DNA detection. ChemComm link |
|
Read more... |
January 27, 2010 Professor Kurt Gothelf receives the EliteForsk award Professor Kurt Vestager Gothelf, Department of Chemistry and iNANO, and director the of the CDNA center, recieves one of five EliteForsk (elite researcher) awards from the Minestry of Science, Technology and innovations. Press release EliteForsk link (in Danish) |
|
|
December 18, 2009 "The Nano box" elected as the top Danish research result of 2009 The Danish engineering journal, Ingeniøren, has elected the DNA nano-box as the research breakthrough of the year 2009 for science and technology. In May 2009, researchers from CDNA, iNANO and University of Göttingen published an article in the journal Nature that described the design and production of the smallest box in the world. News link at AU
|
|
|
More news ...
|
|
|
|
